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Pressure buildup are available in capacities of 100 to 200 L. A special risk to personnel is skin or eye contact with the cryogenic liquid. Cryogenic liquids are materials with boiling points of less than –73 °C (–100 °F). Lab scenes in movies. Self-contained breathing apparatus should be inspected at least once a month and cleaned after each use. When the required heating has been completed, allow the sealed tube or bottle to cool to room temperature. The interval between tests or inspections is determined by the severity of the usage the equipment has received.
Scrubbing or absorbing the gases exiting the pump is also recommended. Completely enclose in a shield to guard against flying glass should the components implode. Make all connections by using, preferably, a plug-and-receptacle combination, or wires with insulated terminals firmly secured to insulated binding posts. 5 Additional Safety Techniques for Equipment Using High Current or High Voltage. At or below freezing temperatures, use the glycerin solution instead of soapy water. Virtually all liquid gases present the threat of poisoning, explosion, or, at a minimum, asphyxiation in a confined space. Use them for immediate first-aid treatment of chemical splashes and for extinguishing clothing fires. Safety first model 2 scenes in the lab answers. • Inspect an AED periodically following the manufacturer's recommendations and procedures as well as after use and before returning to its storage location. 6 Ergonomics and Lifting.
If designed into the fire alarm system, they may automatically sound an alarm and call the fire department, they may trigger an automatic extinguishing system, or they may only serve as a local alarm. The four types of most commonly used extinguishers are listed below, classified by the type of fire for which they are suitable. An eyewash unit provides a soft stream or spray of aerated water for an extended period (15 minutes). Operate Class IIIB and IV lasers only in posted laser-controlled areas. Although the motors in most of the currently marketed stirring and mixing devices meet this criterion, their on/off switches and rheostat-type speed controls can produce an electrical spark any time they are adjusted, because they have exposed contacts. For more information, see Chapter 6, section 6. However, in practice, such modifications are difficult to incorporate into many of the hot plates now in use. As previously noted, use only hot plates that have completely enclosed heating elements in laboratories. In every experimental setup, including temporary ones, use suitable barriers or enclosures to protect against accidental contact with electrical circuits. Aluminum foil, corks, corks wrapped with aluminum foil, and glass stoppers do not meet this criterion, and their use is discouraged.
Joints must allow various sections of the apparatus to be moved if necessary without transmitting strain to the necks of the flasks. Included in this category are fires involving magnesium, lithium, sodium, and potassium; alloys of reactive metals; and metal hydrides, metal alkyls, and other organometallics. In many cases, safety shoes are advisable. Magnetic fields of ~10 G can adversely affect credit cards, watches, and other magnetic objects (see Table 7. Encourage trained laboratory personnel to lease the cylinders and, in essence, only purchase the contents. Do not store new and used cartridges near chemicals because they are constantly filtering the air. • To minimize the possibility of electrical shock, carefully ground the equipment using a suitable flooring material, and install GFCIs. 8 Teflon Tape Applications. There was no other equipment in the oil bath and no other chemicals were in the vicinity. They also may slip off, creating an electrical spark and, perhaps, contacting other metal parts to create an additional hazard. As well as culturing cells and retrieving them from liquid nitrogen, she needs to observe correct laboratory procedure at all times in her role. To flammable liquids or vapors. Stuffing boxes and gland joints are a likely source of trouble in pressure installations. Do not force threads that do not fit smoothly. )
For more information, see the OSHA Personal Protective Equipment Standard (29 CFR § 1910. Regulators for use with noncorrosive gases are usually made of brass. Secure compressed gas cylinders firmly at all times. For example, perform cutting operations with the cutting motion moving away from the body. A pressure gauge is normally a weak point in any pressure system because its measuring element must operate in the elastic zone of the metal involved. Close the cylinder valve as soon as the necessary amount of gas has been released. Metal fatigue will eventually cause any rotor to fail. They are also subject to the limitations of ft. 4. Wear appropriately rated safety glasses, chemical splash goggles, and face shields for eye protection. Keep the sealing surfaces absolutely clean.
It should have a quick-opening valve requiring manual closing; a downward-pull delta bar is satisfactory if long enough. Areas where large amounts of flammable solvents are in use also require explosion-proof lighting and electrical fixtures. 4 Automatic Fire-Extinguishing Systems. It may also cause the magnet to quench, releasing dangerous quantities of gaseous helium into the area. 2 Pressure Reactions in Glass Equipment. A compressed gas is defined as a material in a container with an absolute pressure greater than 276 kPa, or 40 psi at 21 °C or an absolute pressure greater than 717 kPa (104 psi) at 54 °C, or both, or any liquid flammable material having a Reid vapor pressure greater than 276 kPa (40 psi) at 38 °C. Or toxic reactants by using suitable shielding. The vapor that boils off from a liquid can cause the same problems as the liquid itself. This plug combination provides a mechanically and electrically secure connection. Other types of extinguishers should be available if required for the work that will be performed in the laboratory.
In a vertical position, to avoid breakage and injuryWhy shouldn't you hold broken glass with your bare hands? GFCI devices are required by local electrical codes for outdoor receptacles and for selected laboratory receptacles located less than 6 ft (1. Do not use ovens to dry any chemical sample that has even moderate volatility and might pose a hazard. • If clothing is on fire and a safety shower is immediately available, douse the person with water; otherwise, roll the person on the floor to smother the flames. Extra precautions are particularly important for instruments that incorporate high-voltage circuitry. Never tamper with or adapt regulators for use with gases for which they are not designed. Lifting injuries are one of the more common types of injuries for trained laboratory personnel. Do not lower the head into a dry ice chest because carbon dioxide is heavier than air and asphyxiation can result. Design liquid cryogen transfer lines so that liquid cannot be trapped in any nonvented part of the system. Plastic (e. g., polycarbonate) desiccators reduce the risk of implosion and may be preferable but should also be shielded while evacuated. If the device does not bear one of these certification marks, the device should be inspected by an electrician before it is put into service.
See Chapter 6, sections 6. It may be desirable to maintain a log of pump usage as a guide to length of use and potential contaminants in the pump oil. For those solvents that are incompatible with copper (e. g., tetrahydrofuran, methylene chloride, acetonitrile), a second column of alumnia is used along with a dry nitrogen or argon purge to facilitate oxygen removal. Machine can cause cell destruction as well as genetic damage. The useful life of the filter depends on the concentration of contaminant encountered. The preferred source of heat for such vessels is steam, because an explosion in the vicinity of an electrical heater could start a fire and an explosion in a liquid heating bath would scatter hot liquid around the area. The mixture of aluminum powder, heat, and Freon confined in a large airtight area resulted in an explosion. For non-emergency 3 spills, spill control kits may be available. • Dry powder extinguishers, which contain ammonium phosphate or sodium bicarbonate, are effective against burning liquids and electrical fires (Class B and C fires). Because stirring and mixing devices, especially stirring motors and magnetic stirrers, are often operated for fairly long periods without constant attention, consider the consequences of stirrer failure, electrical overload, or blockage of the motion of the stirring impeller. Convenient ways to check for leaks include a flammable gas leak detector (for flammable gases only) or looking for bubbles after application of soapy water or a 50% glycerin–water solution. Bob should notify teacher that glass was broken.
These extinguishers are generally used where large quantities of solvent may be present. Do not let those vessels or equipment made of metals susceptible to amalgamation (e. g., copper, brass, zinc, tin, silver, lead, and gold) come into contact with mercury. Y/NNNever use ch---ed, cr---ed, or d---y ipped, cracked, dirtyAssume glassware is washed before you use it because the person who used it before you washed it for sure. Higher voltages will cause a mantle to overheat, melting the fiberglass insulation and exposing the bare heating element. To avoid electrical hazards, the oven must be grounded. The general procedures discussed in Chapter 6, section 6. To ensure its effective functioning, defrost a freezer manually when ice builds up. The preferred liquids for dry-ice cooling baths are isopropyl alcohol or glycols; add dry ice slowly to the liquid portion of the cooling bath to avoid foaming.