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The pTrc LacZ-Flash expression vector that includes a LacZ ORF with a C-terminal lumio sequence and a 10 his tag, a trp/lac inducible promoter and sequences for enhancing expression of eukaryotic genes in E. Novex sharp prestained protein standard.com. coli. For example, the ratio of the number of residues of a target amino acid to molecular weight may be 4 residues per 10 kDa, or 0. A solution can include one or more buffers, reducing agents, chelators, alcohols, detergents, or dyes. 100 μl of 60 kDa BenchMark™ stock solution (OD=3.

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After electrophoresis the gel was stained with SimplyBlue™ Safe Stain Coomassie G-250® protein stain (Invitrogen Corp., Carlsbad, Calif. ) according to the microwave protocol. In this case, the expressed protein had a molecular weight that was closer to 160 kDa than to the expected 150 kDa. Preferably, reaction conditions that optimize the reaction of the reactive chemical groups of the labeling compound and target amino acid are used for conjugating a selected label to the target amino acid. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. In many cases, fluorophores are also chromophores that have an observable color when they absorb light. 10) was cloned into the AvrII site. The dye was loaded on the C-18 resin in 50 mM phosphate pH 3. Partial selectivity can also be obtained by careful control of the reaction conditions. The set of pre-labeled protein standards of the kit can be provided as lyophilized solids, or in solution in liquid or frozen form. A protein standard selectively labeled on lysine is labeled with a labeling compound that comprises an amino-reactive group, such as, but not limited to, an isothiocyanate, an isocyanate, an acyl azide, an N-hydroxysuccinimide (NHS) ester, a sulfonyl chloride, an aldehyde, a ketone, a glyoxal, an epoxide, an oxirane, a carbonate, an aryl halide, an imidoester, a carbodiimides, or an acid anhydrides. A pre-labeled protein of a standard set of the invention can be made by recombinant methods. For example, a thioredoxin sequence used in a protein standard can have a truncation of from one to 50 amino acids from the carboxy terminus, such as, for example, from one to ten, from ten to twenty, form twenty to thirty, form thirty or forty, or from forty to fifty, amino acids can be truncated from the carboxy terminus. Novex™ Sharp Pre-stained Protein Standard. 42 residues of target amino acid/kDa for a second protein of a standard set, where the first and second proteins have ratios of the number of target amino acid residues to molecular weight that are within 5% of one another. The mixture was stirred thoroughly and then cooled to 0° C. in an ice water bath.

The protein solution plus TCA is incubated at 4° C. for 1-2 hours and then centrifuged at 8, 000×g for 10 minutes at 4° C. The liquid is discarded and 30 ml of ultrapure H2O is added and mixed well. "Target amino acid" refers to an amino acid species, for example lysine, by which is meant all lysine residues of a protein, and is not used to refer to a single particular lysine residue of a protein. Reagents: Complete Protease Inhibitor (Roche Applied Science, Indianapolis, Ind., USA); Freshly prepared 25 mg/ml lysozyme (Calbiochem, San Diego, Calif., USA) in ultrapure water; Induced cell culture as for 30, 40, 50 and 110 kDa (NL) proteins; Amberlite MB-150 (Sigma-Aldrich); Toyopearl AF Chelate 650M (Tosoh Bioscience, Tokyo, Japan); CHAPS detergent; Urea; 1M Na-phosphate pH=7. Activation of Orange 16 Dye. A protein that is depleted in residues of a second amino acid can have no residues of a second amino acid. Novex sharp prestained protein standard edition. Preferably, conjugation to form a covalent bond consists of simply mixing the reactive compounds of the present invention in a suitable solvent in which both the reactive compound and the substance to be conjugated are soluble. Once the product was loaded onto the column the column was washed with 3 column volumes of water and then the product was eluted using 50% HPLC grade methanol in water. Illustrative biological examples include urine, sera, blood plasma, total blood, saliva, tear fluid, cerebrospinal fluid, secretory fluids from nipples and the like. Preferred conservative amino acid substitution groups are: valine-leucine-isoleucine; phenylalanine-tyrosine; lysine-arginine; alanine-valine; glutamic acid-aspartic acid; and asparagine-glutamine. The protein elution was monitored at 280 nm with a UV detector. Preferably, the calculated molecular weights for a pre-labeled protein standard having a molecular weight greater than 5 kDa and its unlabeled counterpart on one of the referenced denaturing acrylamide gels are within 10%, 7%, or 5% of one another. "Conservative amino acid substitutions" refer to the interchangeability of residues having similar side chains.

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Reactions of these groups with a nucleophile-interacting group of a label will be more or less efficient depending on factors that include but are not limited to the reactive group of the label, the strength of the nucleophile group of the amino acid, and the pH at which the reaction occurs. Novex sharp prestained protein standard dual. The flow rate is stopped and the column is incubated for 1 hour at room temperature. For example, the side chains of several amino acids include chemical groups that can act as nucleophiles in chemical conjugation reactions. In certain illustrative examples, the non-target amino acid is capable of reacting with the label more efficiently than any other amino acid in the protein, except for the first amino acid.

8 cm, from the loading site. 1 D3 which had been also digested with XhoI and PmeI. Application||Abreviews||Notes|. The insulin-b chain has theoretical absorbance of 0. After incubation, the excess labeling compound is removed by gel filtration, dialysis, HPLC, precipitation, adsorption on an ion exchange or hydrophobic polymer, or other suitable means. BenchMark™ protein standards are described in U. Please use the form below to provide feedback related to the content on this product. Background Information.

Novex Sharp Prestained Protein Standard.Com

One tablet of inhibitor is used for every 50 ml solution. In making labeled protein standards of the invention, a target amino acid is an amino acid whose labeling is intended; the labeling of a protein on a target amino acid is achieved by selecting a labeling compound with a reactive chemical group that reacts with the reactive chemical group on the target amino acid. "Conjugated to" means covalently bound to. To establish recombinant nucleic acid molecules in cells. A "nontarget amino acid" is an amino acid on a protein standard that has a reactive group that is capable of reacting with a labeling compound conjugated to a target amino acid of the protein standard, but whose conjugation to a labeling compound is not desired. The invention provides molecular weight standard sets in which two or more selectively labeled proteins of different molecular weights comprise different numbers of copies of an amino acid sequence having homology to an amino acid sequence of a naturally-occurring protein. For purification of lysozyme labeled with Uniblue-A, Bio-Gel P-6 column equilibrated with 8M urea was used. The reduction in multiple species of a labeled protein that would otherwise result from this labeling variability provides for more precise separation characteristics. In one embodiment, a protein selectively labeled on lysine comprises two or more copies of an amino acid sequence having 60%, 70%, 80% or greater homology to at least 20, 30, 40, or 50 amino acids of a naturally-occurring protein sequence in which the homologous amino acid sequence of the selectively labeled protein lacks cysteine.

Electrophoretic migration of labeled and unlabeled forms of a protein standard is within a given percentage when the difference in the calculated molecular weights of the labeled and unlabeled forms of the protein using either curve-fitting of molecular weight to migration distances or point-to-point calculation are within the given percentage. In the description that follows, a number of terms used in recombinant DNA technology and protein chemistry are utilized extensively. Numerous fluorophores are known to those skilled in the art and include, but are not limited to coumarin, cyanine, benzofuran, a quinoline, a quinazolinone, an indole, a benzazole, a borapolyazaindacene and xanthenes including fluoroscein, rhodamine and rhodol as well as other fluorophores described in RICHARD P. HAUGLAND, MOLECULAR PROBES HANDBOOK OF FLUORESCENT PROBES AND RESEARCH CHEMICALS (9th edition, CD-ROM, Sep. 2002). The volume of the column was at least 20 times the volume of the sample for proteins labeled with the Red (8-ANS-APVS) dye. The invention provides individual pre-labeled proteins that migrate within 10%, within 7%, within 5%, within 4%, within 2. 50 1M Tris pH=8, 25 ul 20% SDS, and 825 μl ultrapure water were added to 100 μl of a 6. All 7 lysine (K) amino acids were changed to arginine (R) at positions 4, 19, 52, 70, 83 and methionine (M) at position 36 to favor the binding of the dye molecules to cysteine rather than lysine. Journal of Biological Chemistry 269: 15683 (1994)) or a sequence of one or more Bacillus megaterium spore proteins that lack cysteine residues (Setlow, Journal of Biological Chemistry 250: 8168 (1975)). For example, in some embodiments of pre-labeled protein standard sets, one or more selectively labeled protein standards of the set comprises one or more copies of an amino acid sequence that is not known to have homology to a naturally-occurring protein and the one or more selectively labeled proteins is labeled on a first, or target, amino acid and is depleted in a second (non-target) amino acid.

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These methods typically use standards for molecular weight or charge determination. Contaminating bands can interfere with the accurate estimation of protein concentration if total protein concentration in solution is determined. Cysteine and methionine at positions 35 and 37 were replaced with arginine and cysteine to increase the distance between cysteine residues and minimize the potential steric hindrance created by two dye molecules binding to cysteines residues at positions 34 and 37. The sample is allowed to cool down for 5 minutes at room temperature (or until the temperature drops to 30° C. ) and then 5. The expression clone was labeled pTrc 50.

One or more proteins of a set of labeled protein standards can be selectively labeled, for example, on the sulfhydryl group of cysteine, on the primary amine of an N-terminal amino acid and/or the primary amine of lysine, on the secondary amine of the imidazoyl group of histidine or the indole ring of tryptophan, on the carboxyl groups of the C-terminal amino acid or of aspartate or glutamate, on the thioether of methionine, on the phenolate of tyrosine, or on the amidino group of asparagine. A protein standard selectively labeled on lysine is preferably labeled with a dye that comprises an sulfhydryl-reactive group. Electrophoretic Migration. In some embodiments of this aspect of the invention, a selectively labeled protein includes an amino acid sequence having homology to an amino acid sequence of a naturally-occurring protein, in which the naturally-occurring protein is naturally depleted in or deficient in a non-target amino acid.

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An unlabeled standard set comprising the same proteins as the pre-labeled set was also formulated. The column volume was at least ten times the sample volume. 1 forward primer (SEQ ID NO:20) and 50. For example, the protein that is selectively labeled can be a naturally-occurring protein that is isolated from cells, tissue, organisms, biological samples (including fluid samples, such as blood or serum), or media, where at least a portion of the protein naturally has a low abundance of a non-target amino acid. 250 μl of 2 mg/ml 30 kDa (NL) stock solution was brought up to 1 ml volume to a final concentration of 50 mM Tris, 0. In some embodiments of these aspects, one, two, three, four, five, or more than five labeled proteins of a protein standard set having molecular weights of 10 kDa or more are selectively labeled on a target amino acid and migrate substantially the same as their unlabeled counterparts. Fractions were collected (monitored at 280 nm using UV detector). Supplier Catalog Number:||JB-EPL-2500|.

A "recombinant protein" is a protein made from a recombinant nucleic acid molecule or construct. The sample is loaded on the column (about 20 ml of sample can be applied to 100 ml column bed volume). Incubation is at 30 degrees C. for approximately 1. The invention also includes a set of pre-labeled protein standards as in any of the previous embodiments, in which the plurality of labeled proteins are provided in one or more solutions. 30 mL of water was added, followed by 5 mL of 1. 5-fold among the proteins of the set. In this case protein sequences can optionally be selected base on the abundance of cysteine and the paucity of lysine in the amino acid sequence used, which in some embodiments can reduce the number of codons to be mutated. White colonies were selected for colony PCR screening using the specific primer sets used in the cloning. A protein standard selectively labeled on cysteine can optionally be made by recombinant methods from a nucleic acid construct that encodes at least a portion of a sequence of a naturally-occurring protein, in which one or more lysine, histidine, or tryptophan codons has been removed. BlueHeron® Biotechnology (Bothell, Wash., USA) was contracted to synthesize the 1595 bp ORF according to specifications that would allow for optimal protein-dye labeling. 5%, or 1% of one another are selectively labeled on a first amino acid. Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Insert Configuration.

It is believed that during the preparation of the fragments one of the presumed 50 kDa subcloned fragments was a 60 kDa Thio repeat fragment instead of a 50 kDa Thio repeat fragment. Storage bufferpH: 7. Storage: Stable for up to 3 months at 4°C.

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