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Novex Sharp Prestained Protein Standard / Dwarf Fortress Milk Of Lime

02% DTT, 15% Glycerol. PTrc 50 kDa Base Vector: TA clone 50. The yield was calculated by standard methods. The Novex Sharp Pre-Stained Protein Standard is designed for accurate, easy, and convenient molecular weight estimation of a wide range of molecular weight proteins during SDS-PAGE and Western blotting. Reactive Groups of Amino Acids.

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Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. CCGGCGGCCGATGTGTGATCGTATTATTCAT, |50. In some preferred embodiments, a pre-labeled standard set comprises a plurality of labeled proteins, in which at least two of the proteins are selectively labeled on a target amino acid, and the at least two proteins selectively labeled on a target amino acid have ratios of the number of target amino acid residues to molecular weight that are within 5% of one another. Novex sharp prestained protein standard edition. The method includes electrophoresing one or more proteins and at least one prelabeled protein standard set as described herein in a gel; and comparing the migration of the one or more proteins with the migration of least one protein standard of the pre-labeled standard set. Sharp Molecular Weight Marker Expression Plasmids: 110, 160, and 260 kd Proteins. 5, 4% SDS, 60% Glycerol, 0.

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Key product features: - Broad range: 10-245 kDa. The first peak is collected as the protein peak. See all Prestained Protein Ladder reagents. In selecting one or more target amino acids and minimizing labeling of one or more non-target amino acids for labeling a protein standard, the reactivities of the groups present on amino acid side chains are taken into account.

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These products typically do not have pictures or detailed descriptions. In some cases a second purification of a standard protein was performed on Sephacryl column. The sample was loaded on the column and the dye was separated from the protein conjugate. Ab116028 has been referenced in 16 publications. Labeled proteins of a pre-labeled protein standard set on the invention that are not selectively labeled can be recombinant proteins or proteins isolated from cells, tissues, organisms, biological samples, or media. Although reaction conditions can be adjusted to reduce side reactions with one or more amino acids that are not targeted for labeling, side reactions are difficult to completely eliminate or control. In preferred embodiments, the ratios of cysteine residues to molecule weight for the two or more, three or more, four or more, five or more cys-labeled proteins that lack lysine do not vary by more than 5%. As used herein, the terms "about" or "approximately" when referring to any numerical value are intended to mean a value of ±10% of the stated value. Blue Protein Standard, Broad Range, New England Biolabs. A pre-labeled protein of a standard set of the invention can be made by recombinant methods. An exemplary amino acid tag is a His tag.

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5%, or 1% of one another. The pre-labeled protein standards of the present invention are particularly useful in gel electrophoresis, in which molecular weights can be determined using the pre-labeled standards run alongside one or more sample proteins. In the context of the present invention, a first amino acid is an amino acid whose labeling is desired, and whose labeling is targeted by the choice of reactive group on a labeling compound. "Naturally-occurring" refers to the fact that an object having the same composition can be found in nature. This design allowed for the subcloning of this ORF, referred to a BH6mer ORF (SEQ ID NO:13, FIG. Concentration information loading... Novex sharp prestained protein standard version. Research areas. 30, 40, 50 and 110 kDa (no-lysine (NL)) proteins. Proteins can also be made wholly or partly using chemical synthesis. Changing the position of a target amino acid in a protein can be done by altering codons and can be done to improve labeling efficiencies, for example by providing spacing between target amino acids to avoid steric hindrance during the labeling reaction, or to position a target amino acid farther from a charged group, hydrophobic region, etc. In any of these examples an N-terminal amino acid, which can be labeled on the N-terminal amino group, can be a target amino acid or a non-target amino acid. For example, a selectively labeled protein can comprise one or more copies of a sequence from the C-terminus of one or more ADP-ribosylation factors (Schurmann et al.

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In some instances, one or more lysine codons is mutated to a nonlysine codon based on the hydrophilicity, charge, or reactivity of the nonlysine amino acid to optimize properties such as solubility or purification of the labeled protein. Gel electrophoresis in particular is a common tool for the development of new drugs and medical diagnostics that is typically performed with molecular weight markers. The lysis is performed for 1 hour at room temperature on shaker or rotary mixer. The appropriate amount of compound for any protein or other component is conveniently predetermined by experimentation in which variable amounts of the compound are added to the protein, the conjugate is purified (for example, using chromatography) to separate unconjugated compound and the protein-labeling compound conjugate is tested in its desired application. The column was washed thoroughly with water after the dye was loaded. Lane 4: Elite Protein Ladder 10µl. Alkylation was performed at 0. Novex sharp prestained protein standard gold. In some embodiments of these aspects, one, two, three, four, five, or more than five labeled proteins of a protein standard set having molecular weights of 10 kDa or more are selectively labeled on a target amino acid and migrate substantially the same as their unlabeled counterparts. Mass spectrometry analysis of the actual molecular weight of the expressed protein revealed that it was 10 kDa larger than expected (Table 4). The calculated molecular weights of the proteins can be performed by curve-fitting of molecular weight to migration distances or point-to-point calculation. DETAILED DESCRIPTION OF THE INVENTION.

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Ultra-clear and expanded molecular weight range (6. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition. The solution was then cooled back to 0° C. to precipitate the diazonium salt. The pTrc LacZ-Flash expression vector that includes a LacZ ORF with a C-terminal lumio sequence and a 10 his tag, a trp/lac inducible promoter and sequences for enhancing expression of eukaryotic genes in E. coli. 5 residues of the target amino acid per 10 kDa. 36) was brought up to a volume of 1 ml with a final concentration of 50 mM Tris pH=8 and 0. In another embodiment, a pre-labeled protein standard set includes 5 proteins stained with four different dyes having distinguishably different colors, in which the proteins have a molecular weight of from about 20 kDa to about 80 kDa, in which the molecular weights differ of the 5 proteins differ by equal increments, in which the width of bands of the electrophoresed proteins differ by 3% or less.

Novex Sharp Prestained Protein Standard Version

A dye used to label a selectively labeled protein standard of a pre-labeled protein standard set can be a fluorophore. Each of the prestained proteins was loaded side by side with the corresponding unlabeled protein marker on gels. The presence of this valine on the end of the 10 HIS tag did not affect Ni-NTA purification of the synthesized protein. Examples of amino-reactive groups that can be present on a compound used to label lysine, histidine, tryptophan, or an N-terminal amino acid include, but are not limited to, isothiocyanates, isocyanates, acyl azides, N-hydroxysuccinimide (NHS) esters, haloacetyl compounds, maleimide derivatives, sulfonyl chlorides, aldehydes, ketones, glyoxals, epoxides, oxiranes, carbonates, aryl halides, imidoesters, carbodiimides, or acid anhydrides. In one aspect of the invention, a pre-labeled protein standard set includes one or more proteins selectively labeled on a first, or target, amino acid with a labeling compound, in which the one or more selectively labeled proteins is depleted in residues of a second, or non-target, amino acid that is capable of reacting with the labeling compound. Application||Abreviews||Notes|.

In some embodiments, the proteins standards have amino acid tag sequences, such as amino acid tags that can be used to purify the proteins. 913 at 1 mg/ml concentration (according to the Swiss-Prot Protein Parameters tool). Selectively Labeled Protein Standards Comprising an Amino Acid Sequence Derived from a Naturally-Occurring Protein. The term "label" as used herein refers to a chemical moiety or protein that is directly or indirectly detectable (e. g. due to its spectral properties, conformation or activity) when attached to a target or compound and used in the present methods. Protein expression screens in BL21 DE3 STAR were preformed to validate PCR screen screening results. 25 of 20 mg/ml Bodipy 530/550 Iodoacetamide in DMF was added to the protein sample and the sample was incubated for 5-6 hours at room temperature. Codons of a target amino acid can be deleted, inserted, or mutated to codons of other amino acids, for example to provide proteins for labeling that include more than one target amino acid per 10 kDa, such as an average of 2, 3, 4, or more target amino acids per 10 kDa. The 10 kDa BenchMark™ protein marker is the recombinantly-expressed truncated E. coli thioredoxin protein that includes amino acids 1-85 from E. coli thioredoxin, a substitution of glutamic acid for valine at amino acid at amino acid position number 86, and histidine residues at positions 87-92 (Trxfuspr110A; see FIG. Then 50% of the target final volume of 2×Sample Buffer (130 mM Tris pH=6. Please use the form below to provide feedback related to the content on this product. Expression plasmids for the 30, 40, and 50 kDa proteins were made using pTrcBH 60 kd, a construct containing a synthetically derived open reading frame (ORF) consisting of six tandem E. coli thioredoxin (Thio) segments. The sample is vortexed for 10-15 seconds to disperse the pellet and then immediately mixed using a Polytron mixer. An nucleotide-disulfide oxidoreductases can be, as nonlimiting examples, any of SEQ ID NO:1 (E. coli thioredoxin), SEQ ID NO:2 (human thioredoxin), SEQ ID NO:3 (E. coli glutaredoxin 1), SEQ ID NO:3 (E. coli glutaredoxin 2), SEQ ID NO:5 (E. coli glutathione oxidoreductase), SEQ ID NO:6 (human glutathione oxidoreductase), SEQ ID NO:7 (E. coli lipoamide dehydrogenase), SEQ ID NO:8 (human lipoamide dehydrogenase), their variants, their analogues in other species, and variants of such analogues.

2A the six assembled Thio repeats were separated by five unique restriction sites. "Homologous" means that a protein peptide, or amino acid sequence has at least 65%, at least 70% amino acid sequence identity, at least 80% amino acid sequence identity, preferably 90% amino acid sequence identity, and more preferably at least 95% amino acid sequence identity with amino acid sequence referred to. 21, 2007 (abandoned), which claims benefit of priority to U. In certain illustrative examples, the non-target amino acid is capable of reacting with the label more efficiently than any other amino acid in the protein, except for the first amino acid. The invention also includes a set of pre-labeled protein standards that comprises a plurality of labeled proteins, in which one or more of the labeled proteins is selectively labeled on a first amino acid, in which the plurality of labeled proteins are provided in one or more solutions. In some preferred embodiments, the widths of visually detectable bands produced by at least five pre-labeled proteins of a standard set do not differ by more than 30%. The sample is centrifuged for 5 minutes at 5, 000×g to pellet cell debris. Journal of Biological Chemistry 271: 18869-18874 (1996); Yang et al J. Clin. As a nonlimiting example, a pre-labeled protein standard set can comprise from five to twenty labeled proteins, of which from one to twenty are labeled on cysteine and lack lysine residues. The protein contained 73 cysteines and 19 lysine amino acids. 16B depicts a trace extracted from the gel image having peaks 2-13 corresponding to band intensity of the pre-labeled proteins. Similarly, "about 100 mM" (or "approximately 100 mM") encompasses a range of concentrations from 90 mM to 110 mM, inclusive.

5 hours at room temperature.

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