Catalase Enzyme Lab Answer Key Page 2
As the concentration of hydrogen peroxide increases, the rate of the reaction began to increase. Introduction: The introduction provides the reader with background information about the problem and provides the rationale for conducting the research. Hydrogen Peroxide, 3% (at least 100 mL). Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. • Raw liver (fresh or frozen, thawed; one quarter pound). Conical flasks, 100 cm3, x3. Once one of both compounds is depleted, the product formation stops. Catalase enzyme lab answer key quizlet. Catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water. If your results are different from your hypothesis, why might they differ? Similarly, enzymes in the liver are also used to functioning at body temperature (37 degrees Celsius), so microwaving the blended liver to a temperature hotter than that should have damaged the catalase enzyme and clearly decreased the amount of bubbles when it was exposed to hydrogen peroxide.
- Catalase enzyme lab answer key graph
- Catalase enzyme lab answer key quizlet
- Catalase enzyme lab answer key west
Catalase Enzyme Lab Answer Key Graph
The lower the activation energy of a reaction, the faster it takes place. What happens when a potato is combined with hydrogen peroxide? An adult may need to help with this. Learn more: Science Buddies. Exploring Enzymes | STEM Activity. Only small samples of liver, potato and celery are required. What is the dependent variable in the experiment? The given chart and graph show the different concentration levels of the substrate that were used, along with the time it took for the reaction to occur.
In other words, catalase protects cells from the toxic effects of hydrogen peroxide. Sometimes they get weird data, so this helps with accuracy. As the temperature varied from catalases optimal of 37° C, the reaction rate of catalase decreased. The area in which bonds of the reactant(s) are broken is known as the active site. In this lab, students explore how enzymes break down hydrogen peroxide using different catalysts. You have the following materials available: - Test tubes. Critical Thinking With Enzymes. You probably saw lots of bubbles and foam in this activity. Each enzyme has an active site, which is where the reaction takes place (Figure 1). Catalase enzyme lab answer key west. Repeat this experiment, but this time do not add dish soap to all of the reactions. Enzymes are organic catalysts that can affect the rate of a chemical reaction depending on the pH level and the concentration of the enzyme.
Catalase Enzyme Lab Answer Key Quizlet
I've done both and frankly potato is less stinky and is easier to clean up after. Take cup number 1 and place it in front of you on the work area. This suffix indicates that a molecule is an enzyme. You should cite outside resources. Draw another line at the 2 cm mark. Wash the test-tubes. The Liver: Helping Enzymes Help You. This lab explores enzymes using toothpicks. Both plants and animals have peroxisomes with catalase. Try this class experiment to detect the presence of enzymes as they catalyse the decomposition of hydrogen peroxide.
To show the similarity between enzymes and chemical catalysts, the teacher may wish to demonstrate (or ask the class to perform as part of the class experiment) the catalytic decomposition of hydrogen peroxide solution by manganese(IV) oxide (HARMFUL – see CLEAPSS Hazcard HC060). Use the permanent marker to label the remaining 4 cups from 1 to 4. The reactants of enzyme catalyzed reactions are called substrates. This product has a shelf life of 6 months from date of receipt. To cup 1 no further additions are made at this point. This is a resource from the Practical Chemistry project, developed by the Nuffield Foundation and the Royal Society of Chemistry. Since enzymes speed up chemical reactions, without them the reactions would take place way to slowly to actually do your body any good. 494) affects the enzyme more than a pH of 4 (0. Catalase Enzyme Lab Flashcards. • To test the effect of heat, put one teaspoon of the blended liver into a microwave-safe bowl. Other enzymes can also have different effects such as the enzyme in cattle serum.
Catalase Enzyme Lab Answer Key West
If there is no hydrogen peroxide present, the catalase cannot function, which is why in cup 1 you should not have seen any bubble or foam production. Use your original experiment to shape your ideas. Then, the reaction products are released from the pocket and the enzyme is ready to start all over again with another substrate molecule. Additional information. Catalase enzyme lab answer key graph. Hydrogen peroxide is a toxic product of many chemical reactions that occur in living things. A chemical reaction occurs when compounds come together and their molecules interact to form new compounds. The objective is to present the data, not interpret the data. Or you could try adding more than one teaspoon of vinegar or baking soda and then test the enzyme. Communicating Your Findings. IntroductionHave you ever wondered how all the food that you eat gets digested?
Enzymes are essential for life. It also stops the accumulation of cellular organelles. The other two test tubes contained catechol. The name catalase for the enzyme present in all these foodstuffs can be introduced. It should not be a bullet list of steps and materials used; nor should it read like a recipe that the reader is to follow. For this experiment, the saturation point was not reached but could have if the experiment had continued. Can you identify which conditions are optimal for the catalase reaction? You will need to use your scientific method knowledge in today's lab in creating hypotheses for each experiment, devising a protocol to test your hypothesis, and analyzing the results.
Hot plate (for boiling water). These should be general, not specific, statements that are possible answers to your questions. Here is the gist of the lab: - Students will need: potato puree, tweezers, a beaker full of hydrogen peroxide, and a stopwatch. Place the paper in the bottom of the beaker of peroxide and start the stopwatch. The answer is, when the enzyme catalase comes in contact with its substrate, hydrogen peroxide, it starts breaking it down into water and oxygen. Prepare a bar graph of catalase activity. What happens to catalase itself in the reaction – is it used up?
Be careful of the sharp blades in the blender. This is an easy assignment that encourages students to think about what they know about enzymes and apply their knowledge to real-world scenarios. Enzyme Function: Penny Matching. Catalase is an enzyme in the liver that breaks down harmful hydrogen peroxide into oxygen and water. Ruler and wax pencil. The enzyme catalase can speed up (catalyse) this reaction. If you have any additional questions, leave me a comment! In fact, the catalase reaction is dependent on the substrate concentration. You are already familiar with the steps of the scientific method from previous lab experiences. You should have seen more foam being produced once you added another tablespoon of hydrogen peroxide to cup 1, which should have resulted in a similar amount of foam as in cup 3. Students will view the penny machine in action and then compare this process to an enzyme-catalyzed reaction.